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2 edition of Regulation of trout sperm motility found in the catalog.

Regulation of trout sperm motility

Scott Anthony Boitano

Regulation of trout sperm motility

evaluation of swimming parameters and the role of membrane potential, pH, and Ca⁺⁺ in activation and regulation of motility

by Scott Anthony Boitano

  • 156 Want to read
  • 23 Currently reading

Published .
Written in English

  • Spermatozoa -- Motility.,
  • Fishes -- Spermatozoa.,
  • Trout.

  • Edition Notes

    Statementby Scott Anthony Boitano.
    The Physical Object
    Paginationix, 107 p. :
    Number of Pages107
    ID Numbers
    Open LibraryOL16894276M

    Eukaryotic cilia and flagella are vital sensory and motile organelles. The calcium channel PKD2 mediates sensory perception on cilia and flagella, and defects in this can contribute to ciliopathic diseases. Signaling from Pkd2-dependent Ca(2)+ rise in the cilium to downstream effectors may require intermediary proteins that are largely unknown. To identify these proteins, we carried out Cited by: CatSper2, is a protein which in humans is encoded by the CATSPER2 gene. CatSper2 is a member of the cation channels of sperm family of protein. The four proteins in this family together form a Ca 2+-permeant ion channel specific essential for the correct function of sperm cells.. Function. Calcium ions play a primary role in the regulation of sperm s: CATSPER2, CatSper2, cation channel sperm .

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Regulation of trout sperm motility by Scott Anthony Boitano Download PDF EPUB FB2

Morisawa M, Okuno M () Cyclic AMP induces maturation of trout sperm axoneme to initiate motility. Nature – PubMed CrossRef Google Scholar Morisawa M, Suzuki K, Shimizu H, Morisawa S, Yasuda K (a) Effects of osmolality and potassium on motility of spermatozoa from freshwater cyprinid by: 2.

The physiological regulation of sperm motility has become more amendable to investigation since the demonstration that cAMP and calcium play a role in modulating the functioning of the flagellar axoneme. Although the external triggering mechanisms that initiate motility and capacitation are still unknown.

Cell signalling for the initiation of sperm motility in the salmonid fish has drawn much attention during the last two decades. In some species, protein phosphorylation process was shown to be involved in flagellar motility regulation. Hyperpolarization of the sperm membrane induces synthesis of cAMP.

In conclusion, the results in the present work indicated that water influx across plasma membrane plays a crucial role in the regulation of salmonid fish sperm motility.

The water influx across the plasma membrane maintains sperm motility in intact sperm, and it initiates sperm motility in glycerol-treated by: Abstract. Changes in the motility time of spermatozoa collected from the testes and the sperm duct of normal and sex-reversed male (XX) rainbow trout in physiological balanced salt solution were examined after incubation in artificial seminal plasmas of various pHs.

Although untreated spermatozoa from the sperm duct retained motility Cited by: transduction in the sperm. The best characterized factors for the signal cascade in the regulation of sperm motility are cAMP and Ca2+ (Walczak and Nelson, ): cAMP-dependent phosphorylations of axonemal proteins have been reported to regulate the motility of sperm in salmonid fish (Morisawa and Okuno, ), seaCited by: With regard to Salmonidae, irradiated sperm of rainbow trout showed approximately 60% of motility after a minute exposure to UV irradiation, became motile and maintained progressive movements.

The male reproductive organ of rainbow trout and chum salmon consists of a pair of testes and sperm ducts. Spermatozoa in the distal portion of the sperm ducts exhibit full motility in the K+-free medium. However, spermatozoa from the testis were almost immotile in this medium.

This suggests that the spermatozoa acquire a capacity for movement during their passage from the testis along the. Publisher Summary This chapter focuses on the regulation of sperm ion currents—that is, the participation of the sperm ion channels in the information.

Motility is an important function of the male gamete, which allows sperm to actively reach and penetrate the female gamete in organisms with internal and external fertilization. Sexual activity of some fish is generally seasonal and fertilization is external.

Abstract. Steelhead trout Oncorhynchus mykiss sperm held in seminal plasma or sperm-immobilizing buffer (pH 86) at 10° C consumed O 2 at the rate of c. 2 nmol O 2 min −1 10 −9 sperm; the rate of O 2 consumption was not different in sperm held for 4 or 24 h.

Decreasing the extracellular pH from 85 to 75 either by diluting semen with buffer titrated with HCl or by increasing the. The objective measurement of fish sperm motility was first reported by Cosson et al.

() using stroboscopic illumination and video recording of activated trout sperm movement. Only during the last few years have modern CASA systems been adapted to fish spermatozoa studies Toth et al.,Toth et al.,Christ et al.,Kime et Cited by: This study investigated the relationships between semen fertilization capacity and sperm motility, seminal plasma composition and sperm metabolism in the rainbow trout, Oncorhynchus mykiss, to find out biomarkers for semen ions in semen fertilization rate could be best described by three multiple regression models: Firstly, a model including the seminal plasma pH (x 1), β-d Cited by: This book is comprised of three parts encompassing 15 chapters.

Part I explores the ability of egg factors to affect sperm motility and initiate the acrosome reaction by modifying ion movements across the sperm plasma membrane. Ionic regulation of sea urchin sperm motility, metabolism and fertilizing capacity.

Abstract. In order to pursue the significance of the ionic regulation of sea urchin sperm behaviour, alterations in the cation composition of sea water were tested for their effects on sperm fertilizing by: During the period after spermatozoa comes in contact with an activating medium, the ion concentrations inside the sperm cell are rebalanced, and osmotic pressure affecting the sperm membrane becomes harmful for sperm integrity, limiting the period of motility to a short by: 1.

Email your librarian or administrator to recommend adding this book to your organisation's collection. Textbook of Clinical Embryology. Control of sperm motility and fertility: diverse factors and common mechanisms.

Role and regulation of PI3K in sperm capacitation and the acrosome : Junaid Kashir, Celine Jones, John Parrington, Kevin Coward. Several events important for successful fertilization in many species rely on adequate sperm motility—namely, (1) Penetration of the extracellular matrix surrounding eggs, (2) directed motility in response to factors released from the egg or closely associated structures, and, (3) migration through the female reproductive tract or within an.

Adenylylcyclase (AC) from sea urchin sperm does not appear to be regulated by G proteins [Hildebrandt, J. D., Tash, J.

S., Kirchick, H. J., Lipschunits, L., Secra, R. D., & Birmbaumer, L. () Endocrinology−]. During sperm activation and the acrosome reaction, membrane potential changes and cAMP increases. Here we explore if membrane potential can modulate the sperm AC Cited by: Sperm forward motility is an essential parameter in mammalian fertilization.

Studies from our laboratory have identified and characterized a few unique sperm motility regulatory proteins/glycoproteins from the male reproductive fluids and mammalian blood serum. The purified sperm motility-initiating protein (MIP) from caprine epididymal plasma as well as the forward motility-stimulating factor Cited by: 2.

SPERM OXIDASE AND SOURCES OF REACTIVE OXYGEN SPECIES FOR SPERM CAPACITATION. The identity of the sperm oxidase involved in capacitation remains elusive. Importantly, it is not clear whether the same enzyme is responsible for generating both O 2 •– and NO • during capacitation, depending on the species under study.

of the activation signal. These trans-activated sperm become motile and capable of fertilization, indicating the importance of motility for sperm cell function. Motility and MSP Video microscopy of crawling sperm (Movie 1) reveals the critical role of MSP polymerization in cell motility (Roberts and Ward, ; Sepsenwol et al., ).

Initiation of sperm motility is uniquely regulated in different species and this regulation is dependent on the environment in which fertilization occurs.

Several factors have been reported to regulate sperm motility; for example, in mammals, bicarbonate and calcium present in seminal plasma are essential to regulate this process (Morisawa, ; Okamura et al., ).Cited by: Motility is an important function of the male gamete, which allows sperm to actively reach and penetrate the female gamete in organisms with internal and external fertilization.

Sexual activity of some fish is generally seasonal and fertilization is external. Sperm, once differentiated in the gonad, remain there completely quiescent until they are released into the external medium, which is. A critical assessment of the response to caffeine of human sperm motility.

Fertility and Sterility37 (3), DOI: /S(16) Masaaki Morisawa, Makoto Okuno. Cyclic AMP induces maturation of trout sperm axoneme to initiate motility. In summary, uric acid contributes to preserving and enhancing sperm motility, viability, and morphology, which in turn protects sperm function.

This contribution is achieved principally by counteracting the damaging effect of oxidizing (e.g., reactive oxygen Cited by: 5. Inhibition of sperm motility in neutral and acidic pH conditions (≤) demonstrated that the low pH of the gonadal environment (pH ) maintains sperm in the quiescent stage.

The inhibitory role of acidic pH in sperm motility was also suggested in the black-lip pearl oyster, characterized by an optimal range of pH values for sperm motility ranging from to (Demoy-Schneider et al., ).Cited by: 9. The movement of live trout spermatozoa is very brief (25 sec at 20 degrees C) and conditions have been developed to get synchronous initiation of sperm motility which allowed quantification of the major parameters of sperm movement during the motility by:   In rainbow trout sperm, the initiation of sperm motility is concomitant with cAMP-dependent phosphorylation of a kDa protein on tyrosine residues.

In Ciona, sperm motility is stimulated by cAMP but inhibited by a PTK inhibitor [ 47 ].Cited by: (). Phosphoproteins associated with cyclic nucleotide stimulation of ciliary motility in Paramecium. Phosphorylation of a 15K axonemal protein is the trigger initiating trout sperm motility.

Preparation and purification of dynein. Protein phosphorylation: the second messenger signal transducer of flagellar motility Author: Chinmoy S. Dey and Charles J. Brokaw. Trout sperm motility is inhibited by high extracellular [K + ] and can be activated by dilution of extracellular [K + ].

Activation of trout sperm by the dilution of extracellular [K Cited by: Non-random chromosome positioning in mammalian sperm nuclei, with migration of the sex chromosomes during late spermatogenesis.

J Cell Sci (): – Author: William Holt, Jane Morrell. Regulation of protein phosphorylation and motility of sperm by cyclic adenosine monophosphate and calcium. Regulation of sperm flagellar motility by calcium and cAMP-dependent phosphorylation. Regulation of sperm flagellar movement by protein phosphorylation a d dephosphorylation.

Cell Motility and the Cytoskeleton. ().Author: Charles J. Brokaw. For example, a woman may produce normal eggs, but the eggs cannot reach the uterus because the uterine tubes are blocked or otherwise compromised. A man may have a low sperm count, low sperm motility, sperm with an unusually high percentage of morphological abnormalities, or sperm that are incapable of penetrating the zona pellucida of an egg.

The effects of different carbon dioxide (CO2) levels on the short-term storage of semen samples from hatchery-produced steelhead (Oncorhynchus mykiss) were evaluated. Sperm motility and fertilizing ability were significantly reduced following 4 h incubation under a relatively modest (≥ kPa = 1%) amount of CO2.

The dose-dependent reductions, however, were not the result of cell death as Cited by:   Moreover, sperm recovered from cftr deficient mice showed significantly reduced motility and fertilizing capacity (Xu et al., ).

These findings indicate that CFTR is involved in HCO 3 − entry into the sperm, which is essential to capacitation. The involvement of CFTR in capacitation has also been confirmed in human sperm (Li et al., ).Cited by: Initiation of movement, the motility period and the arrest of motility of fish spermatozoa allow to develop specific studies on general understanding of regulation and signaling of sperm motility in terms of flagellar beating and wave parameters, thus leading to a better acquaintance of the fine-tuning of the internal axonemal mechanics (see Cited by: 1.

But, so far, none of these ion channels has been linked to the regulation of sperm motility. That changes with an impressive paper by Ren and colleagues 2, Cited by: From this auspicious beginning, the book then proceeds to cover the unique structure of sperm chromatin, the development and use of genomic and proteomic technologies in human sperm assessment, sperm maturation in the epididymis, regulation of sperm motility, interaction of sperm with reactive oxygen species, interaction of sperm with the egg Author: Bayard T.

Storey. The sodium-proton membrane exchange mechanism can modulate pHi and affect motility (26). The pHi changes are also involved in sperm motility as the sperm cell passes through the epididymis (27, 28).

Also, the pH of the epididymal cauda fluid is acidic in almost all species (29) and acts directly on the pHi to decrease the sperm motility (28). J Sperm motility is the major decisive factor in determining male fertility. The objective of the present study was to analyse the effect of mitochondrial membrane potential (MMP) on the temporal regulation of sperm motility.Catsper1 plays an important role in evoked Ca 2+ entry and regulation of hyperactivation in sperm.

Catsper2 is localized in the sperm tail and is responsible for regulation of hyperactivation. Catsper3 and Catsper4 are found in both, the testes and sperm and play an important role in the motility of hyperactivated ro: IPR  Values of progressive motility (a + b grade, expressed as a percentage of the total) in sperm sample C (initial progressive motility between 30 and 45% of total) were measured 5 min before (initial value) and after incubation of the sperm sample for different times with an agonist or the corresponding by: